Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tet1

Cell type

Cell type Class
Neural
Cell type
Frontal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Egr1KO_TET1_5D6
strain background
C57BL/6
genotype/variation
Egr1KO
age
6-week old
Sex
male
tissue/cell type
frontal cortex
chip antibody
TET1 (Active Motif, 61741)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue pellets were homogenized in lysis buffer. After clarifying the lysate from sonicated nuclei, the genomic DNA fragments were obtained from anti-TET1 immunoprecipitated chromatin Libraries were prepared according to the protocol developed by Blecher-Gonen et al. (PMID: 23429716)

Sequencing Platform

instrument_model
Illumina HiSeq 4000

mm10

Number of total reads
81692636
Reads aligned (%)
85.5
Duplicates removed (%)
15.5
Number of peaks
1019 (qval < 1E-05)

mm9

Number of total reads
81692636
Reads aligned (%)
85.5
Duplicates removed (%)
15.4
Number of peaks
1078 (qval < 1E-05)

Base call quality data from DBCLS SRA